PA4-Degrading Enzyme Data: Expression and Structural Characterization from Soil Bacterium

1.7 KB of data supporting the heterologous expression and structural characterization of a polyamide 4-degrading enzyme (Nyl4A_px) from the soil bacterium Pseudoxanthomonas sp. TN-N1. The dataset, authored by Yusuke Saito and last updated in June 2026, details enzyme activity measurements and structural domain predictions. It represents the first reported structural analysis of a PA4-degrading enzyme from a terrestrial source.

Use Cases

• Compare enzyme activity levels based on the reported 68.8 Δ655 nm/h/100 mL broth measurement from recombinant Brevibacillus choshinensis.
• Analyze structural domain predictions (substrate-binding, middle, catalytic) for homology modeling with marine enzyme Nyl4A_pa.
• Benchmark heterologous expression systems using the described 2.4-fold activity increase between E. coli and B. choshinensis hosts.

Strengths

• Dataset is openly licensed under CC-BY-4.0.
• Provides specific enzyme activity measurements (68.8 Δ655 nm/h/100 mL broth).
• Describes a novel, first-reported enzyme structure from a soil bacterium.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (1.7 KB), indicating limited scope.

Provenance

Source

figshare

Collection Method

Experimental data from heterologous expression and structural bioinformatics analysis.

Freshness

Last updated 2026-06-02 05:40:33; freshness should be verified.