HMGB1 Regulation of Mitochondria and ROS in Mouse Embryonic Stem Cells

A 7.3 MB dataset from figshare, last updated May 12, 2026, containing data related to the role of HMGB1 in mouse embryonic stem cells. The data was generated by Tatiana Y. Starkova and investigates mitochondrial structure and reactive oxygen species balance during the transition from naïve to primed pluripotency. It includes results from CRISPR/Cas9 knockout experiments, RNA sequencing, flow cytometry, and transmission electron microscopy.

Use Cases

• Modeling the relationship between chromatin proteins and mitochondrial function based on HMGB1 knockout data.
• Analyzing transcriptomic changes during pluripotency transition based on RNA sequencing results.
• Studying the impact of reactive oxygen species on cell proliferation based on flow cytometry measurements.
• Investigating ultrastructural changes in mitochondria based on transmission electron microscopy images.

Strengths

• Data is derived from multiple experimental methods including RNA sequencing, flow cytometry, and transmission electron microscopy.
• The dataset has a specific biological focus on the HMGB1 protein's role in a defined cellular transition.
• It is shared under a permissive CC-BY-4.0 license for reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small at 7.3 MB, potentially limiting its scope.

Provenance

Source

figshare

Collection Method

Generated from laboratory experiments on mouse embryonic stem cells, including CRISPR/Cas9 knockout, molecular assays, and microscopy.

Freshness

Last updated 2026-05-12 05:43:11; freshness should be verified.