NK-92 Cell Cytotoxicity Data from Membrane-Stirred Bioreactor Study

Figshare hosts a 577.8 KB PDF containing experimental data on NK-92 cell expansion and cytotoxicity. The study by Valentin von Werz compares a membrane-based stirring system to a conventional pitched-blade impeller in 2 L bioreactors. It reports that cells from the membrane-stirred system exhibited higher cytotoxicity, linked via computational fluid dynamics to more homogeneous shear distribution.

Use Cases

• Compare cytotoxicity and cytotoxic capacity metrics between membrane-stirred and pitched-blade impeller bioreactor systems.
• Analyze growth, viability, and metabolic profile data from NK-92 cells cultured under fed-batch conditions.
• Use computational fluid dynamics simulation results to correlate shear force distribution with observed cell performance outcomes.
• Evaluate bioreactor scalability for allogeneic cell therapy production using data from this 2 L stirred-tank platform study.

Strengths

• Data is derived from a controlled, comparative experiment in 2 L bioreactors, a clinically relevant scale.
• The study provides a direct link between bioreactor engineering (shear force distribution) and a key functional cell output (cytotoxicity).
• Dataset is openly available under a CC BY 4.0 license, facilitating reuse.

Limitations

• The core data is embedded within a 577.8 KB PDF, requiring extraction for computational analysis, which may introduce errors.
• The dataset scope is limited to a single cell line (NK-92) and a specific 2 L bioreactor setup, limiting generalizability.
• Sample sizes, replicate counts, and raw numerical data tables are not explicitly stated in the provided description.

Provenance

Source

Valentin von Werz via figshare.

Collection Method

Experimental data from comparative cultivation of NK-92 cells in two different 2 L stirred-tank bioreactor systems, supplemented by computational fluid dynamics simulations.

Freshness

Last updated on 2026-03-23.