Human Biliary Atresia Extrahepatic Cholangiocyte Organoids Transcriptomic Data

Adi Har-Zahav's dataset contains transcriptomic profiling data from patient-derived extrahepatic cholangiocyte organoids (EHCOs) generated from bile duct remnants of biliary atresia (BA) patients and controls. The data includes bulk RNA-seq results and targeted molecular and functional validation, identifying dysregulation of ER stress pathways, xenobiotic metabolism, and genes regulating cell adhesion and polarity. The dataset was last updated on May 7, 2026.

Use Cases

• Identify dysregulated ER stress pathway genes based on transcriptomic profiling.
• Analyze alterations in xenobiotic and drug metabolism gene expression.
• Investigate changes in cell adhesion and polarity markers like E-cadherin and RhoU.
• Validate findings from organoid models using immunostaining data from liver hilum biopsies.

Strengths

• Data is derived from patient-specific organoid models, providing a disease-relevant biological system.
• Analysis includes both discovery bulk RNA-seq and targeted molecular validation.
• Functional validation includes susceptibility to toxic injury and CYP450 inhibition experiments.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is a single PDF file of 244.4 KB, indicating a limited scope of primary data.

Provenance

Source

figshare

Collection Method

Patient-derived organoid generation followed by bulk RNA-seq and molecular validation.

Freshness

Last updated 2026-05-07 04:37:36; freshness should be verified.