Shared APC Neoantigen Data for Self-Amplifying RNA Cancer Vaccine Proof-of-Concept

A research paper detailing the identification and in vivo testing of a shared neoantigen for colorectal cancer vaccination. The study utilized bioinformatics to identify epitopes from the mutational cluster region of the APC gene in multiple patients. It describes the development of a virus-like particle to deliver a self-amplifying RNA replicon encoding the neoantigen and presents results from in vitro T-cell assays and in vivo mouse model experiments.

Use Cases

• Identify shared neoantigen epitopes based on mutations in the APC gene described in the paper.
• Design cancer vaccine constructs based on the described chimeric virus-like particle (VLP) and self-amplifying RNA (saRNA) replicon platform.
• Benchmark T-cell activation assays using the in vitro methods for neoantigen peptide fragments.
• Evaluate in vivo immune responses by measuring neoantigen-specific IgG titers in serum samples from vaccinated mice.

Strengths

• The study provides a specific proof-of-concept for a vaccine targeting a mutation present in over 80% of colorectal cancer cases.
• Results include both in vitro T-cell activation data and in vivo antibody titer measurements from mouse models.
• The dataset is openly licensed under CC-BY-4.0.

Limitations

• The dataset is a single 300.8 KB PDF file; the underlying raw data (e.g., sequence files, assay results) are not provided.
• Row count and column-level documentation are unknown, limiting direct analysis.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Bioinformatics analysis and experimental validation described in the research paper.

Freshness

Last updated 2026-06-04 05:19:33; freshness should be verified.