Transcriptomic Profiles of Mouse Cortical Neurons in Primary Cultures

Seulkee Yang analyzed primary cortical cultures to compare their transcriptional profiles to multiple in vivo mouse cortical reference datasets. The 2.6 MB PDF resource, last updated in May 2026, highlights transcriptional divergence associated with metabolic and physiological state when cells lack native tissue architecture. It provides a resource for understanding how neuronal transcriptional programs are reshaped in vitro.

Use Cases

• Benchmarking cell type classification tools based on transcriptomic profiles from in vitro cultures.
• Investigating the influence of structural cues on neuronal maturation based on incomplete canonical marker profiles.
• Studying transcriptional divergence in metabolic and physiological states when cells are removed from their native environment.
• Comparing in vivo and in vitro transcriptional signatures for major neuronal subclasses.

Strengths

• The analysis compares transcriptional profiles to multiple in vivo mouse cortical reference datasets.
• The resource is a 2.6 MB PDF file with a clear, CC-BY-4.0 license.
• The findings highlight specific transcriptional divergence associated with metabolic and physiological state.

Limitations

• The dataset is a PDF document; the underlying tabular or raw transcriptomic data is not directly provided.
• Column-level documentation and sample data are unavailable, limiting direct analysis of the underlying data.
• Row count is unknown, which may limit suitability assessment for computational tasks.

Provenance

Source

figshare

Collection Method

Analysis of primary cortical cultures compared to in vivo mouse cortical reference datasets.

Freshness

Last updated 2026-05-05 04:13:31