Data Sheet 1_Immune dysregulation in gestational diabetes mellitus: placental downregulati

A study of immune-related gene expression and immune cell infiltration in placentas from pregnancies with gestational diabetes mellitus (GDM). The dataset includes mRNA transcript profiles from the GSE70493 dataset and validation data from an independent cohort of 50 GDM and 50 non-GDM pregnancies. It was authored by Zhimei Zhou and last updated on 2026-05-29.

Use Cases

• Identify differentially expressed immune-related genes (DIRGs) in GDM placentas based on transcriptomic data.
• Analyze immune cell infiltration patterns, such as monocytes and T cells, using CIBERSORT-derived estimates.
• Validate candidate gene expression (e.g., CXCL9, IL1RL1) through qRT-PCR and immunohistochemistry data.
• Perform functional enrichment analysis on immune-related pathways like Th1/Th2 cell differentiation.

Strengths

• Includes an independent validation cohort of 100 samples (50 GDM, 50 Non-GDM).
• Validation performed using multiple methods (qRT-PCR and immunohistochemistry).
• Effect sizes (Cohen’s d) and statistical significance (p-values) are reported for key findings.
• Released under a permissive CC-BY-4.0 license.

Limitations

• Row count and column-level documentation are unknown, requiring manual inspection after download.
• The discovery dataset had a sample size of 55 (30 GDM, 25 Non-GDM) after technical replicate correction.
• The authors note the discovery dataset did not support the use of identified genes as diagnostic biomarkers.

Provenance

Source

figshare, authored by Zhimei Zhou.

Collection Method

mRNA transcript expression profiles retrieved from the public GSE70493 dataset and integrated with immune-related genes from the ImmPort database.

Freshness

Last updated 2026-05-29 06:22:22; freshness should be verified.