Data Sheet 1: In Vivo CRISPRa for Cardiac Fibroblast Reprogramming in Mice

A 627.1 KB PDF document details a CRISPR activation study targeting cardiac fibroblasts in transgenic mice. Author Suchandrima Dutta published the work on figshare under a CC-BY-4.0 license, with a last update timestamp of 2026-04-22. The research demonstrates in vivo reprogramming of fibroblasts into cardiovascular cell lineages following myocardial infarction.

Use Cases

• Analyze CRISPRa-mediated gene activation patterns based on the described Gata4, Nkx2.5, Tbx5, Isl1, and Smarcd3 (GNTIS) targets.
• Study cardiac fibroblast plasticity and transdifferentiation based on the described in vivo mouse model following myocardial infarction.
• Evaluate hemodynamic and functional cardiac parameters based on the results from the described dCas9 transgenic mouse experiments.

Strengths

• The dataset is a detailed research document describing a specific CRISPR activation (CRISPRa) methodology.
• The file is licensed under CC-BY-4.0, permitting broad reuse with attribution.
• The description provides a structured scientific background, methods, results, and conclusion.

Limitations

• The primary data format is a PDF; underlying raw experimental data (e.g., columnar data) is not directly accessible.
• Row count and column-level documentation are absent, limiting suitability assessment for computational analysis.
• Data may reflect the specific experimental bias inherent to the described transgenic mouse model and myocardial infarction protocol.

Provenance

Source

figshare

Collection Method

Likely contains experimental data from in vivo CRISPR activation studies in transgenic mice.

Freshness

Last updated 2026-04-22 05:49:27; freshness should be verified.