In Vivo Lung Microbiome Alterations from Burn Pit and Sand Inhalation Exposures

A dataset of bacterial amplicon sequence variants from the lung microbiomes of Sprague–Dawley rats exposed to burn pit emissions, sand, or a combination. The data was generated by Jeanette S. Frey via 16S rRNA gene sequencing of bronchoalveolar lavage fluid collected at 4 and 90 days post-exposure. The dataset was last updated on April 20, 2026.

Use Cases

• Identify microbial biomarkers for burn pit exposure based on altered genera like Bradyrhizobium and Methylobacterium.
• Analyze longitudinal changes in lung microbiome diversity at 4-day and 90-day post-exposure time points.
• Compare the effects of combined exposures (BPE + Sand) versus single exposures on microbiome composition.
• Investigate the role of specific genera (e.g., Corynebacterium, Geobacillus) in early response to inhalation exposures.

Strengths

• Data includes a controlled in vivo exposure scenario with clean air, BPE, Sand, and combined exposure groups.
• Sequencing results capture microbiome changes at two distinct time points: 4 days and 90 days post-exposure.
• Findings are detailed in a published methods section describing genomic DNA extraction and 16S rRNA gene sequencing.

Limitations

• The dataset is small at 732.0 KB, indicating limited scope.
• Row count and column-level documentation are unknown, which may limit suitability assessment.
• Data may reflect bias inherent to the specific animal model and exposure chamber setup used.

Provenance

Source

Jeanette S. Frey via figshare

Collection Method

Bronchoalveolar lavage fluid was collected from Sprague–Dawley rats after whole-body exposure chamber experiments; bacterial ASVs were identified via 16S rRNA gene sequencing.

Time Range

Samples collected at 4 days and 90 days post-exposure.

Freshness

Last updated 2026-04-20 10:30:09