Data Sheet 1: Inhibition of GPR68 in Diffuse Intrinsic Pontine Glioma Cells

Leif R. Neitzel published a research data sheet on figshare on May 13, 2026, detailing experiments on GPR68 inhibition in Diffuse Intrinsic Pontine Glioma (DIPG). The 526.4 KB PDF describes methods including CellTiter-Glo viability assays, ferroptosis marker measurements, and genetic validation using shRNA knockdown and overexpression. Results indicate that GPR68 inhibition induces ATF4-dependent ferroptotic cell death in DIPG cell lines.

Use Cases

• Validate GPR68 as a therapeutic target for DIPG based on described cell viability assays.
• Study ferroptosis induction in cancer cell lines based on described markers like glutathione levels and lipid peroxidation.
• Compare drug sensitivity between DIPG and normal human astrocytes based on the reported LD50 values.
• Investigate the role of ATF4 and SLC7A11 in ferroptosis signaling based on the described qRT-PCR and overexpression experiments.

Strengths

• Includes specific LD50 values for drug sensitivity (e.g., NHAs: 3.84 µM, DIPG cells: ≤ 0.79 µM).
• Describes a multi-method validation approach including viability assays, ferroptosis marker measurement, and genetic rescue experiments.
• Explicitly compares results across three DIPG cell lines (SF7761, DIPG36, DIPG007) and normal human astrocytes.

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

Leif R. Neitzel

Collection Method

Experimental research involving cell culture, viability assays, and molecular biology techniques as described.

Freshness

Last updated 2026-05-13 04:48:15; freshness should be verified.