Ocimum Sanctum Nanoemulsion Bioactivity Data from Metabolomics and Pharmacology Study

Surfacing results from a study characterizing an Ocimum sanctum nanoemulsion, including physicochemical properties and multi-bioactivity assays. It includes data on droplet size (51–73 nm), cytotoxicity IC50 values against four cancer cell lines (13–25 μg/mL), and antioxidant SC50 values (16.4 μg/mL). The data was generated by Mervat S. Mohamed and published on figshare in 2026.

Use Cases

• Analyze the relationship between nanoemulsion droplet size, polydispersity index (PDI), and zeta potential on cytotoxicity IC50 values across Caco-2, HepG2, MDA-MB-231, and A549 cell lines.
• Compare the antibacterial inhibition zones and antioxidant SC50 values of the nanoemulsion against the crude extract to quantify bioactivity enhancement.
• Validate network pharmacology predictions by correlating identified metabolite profiles with experimentally measured reductions in TNF-α and IL-6 secretion in LPS-stimulated RAW 264.7 macrophages.
• Model the biphasic release profile of the nanoemulsion over eight days against its cytotoxic potency.

Strengths

• Includes multi-modal bioactivity data: cytotoxicity (4 cancer cell lines), antibacterial, antioxidant, and anti-inflammatory assays.
• Reports specific, quantified physicochemical properties: droplet size range (51–73 nm), PDI (0.264), zeta potential (-42.1 mV), and entrapment efficiency (96.2 ± 3.1%).
• Compares nanoemulsion performance directly to a crude extract baseline for key metrics like IC50 and SC50.

Limitations

• Dataset is small (3.8 MB PDF), likely containing summarized results rather than raw, high-volume experimental data.
• Study is preliminary and in vitro; results require validation through more extensive studies and in vivo evaluation as noted by the authors.
• No raw columnar or sample data is available; analysis is confined to the figures and tables within the published PDF.

Provenance

Source

figshare, authored by Mervat S. Mohamed.

Collection Method

Experimental study using GC–MS and LC–QTOF–MS/MS metabolomic profiling, nanoemulsion preparation via high-pressure homogenization, and in vitro bioactivity assays.

Freshness

Last updated 2026-03-20.