Integrative Analysis of Gut Microbiota and Pharmacology in APEC-Challenged Broilers

A research document analyzes the protective effects of Sihuang Zhili Granules against Avian Pathogenic Escherichia coli O78 in broilers. The study combines 16S rRNA gene sequencing of cecal microbiota with network pharmacology and molecular docking analyses. The document was authored by Zikai Guo and last updated in May 2026.

Use Cases

• Study gut microbiota changes under pathogen challenge based on 16S rRNA sequencing results
• Investigate potential drug mechanisms via network pharmacology based on candidate compounds like eupatorin and oroxylin A
• Analyze correlations between cytokine levels and gut barrier gene expression based on described phenotypic and molecular data
• Model host-pathogen-drug interactions based on the integrative analysis framework described

Strengths

• The description provides specific phenotypic outcomes, including reduced mortality and improved histopathology.
• It details specific molecular findings, such as changes in mRNA expression of Occludin and Claudin-3 genes and serum levels of IL-1β, IL-6, and TNF-α.
• The analysis integrates multiple methodologies: microbiota sequencing, network pharmacology, and molecular docking.

Limitations

• The primary data file is a 7.6 MB DOCX document; the underlying raw sequencing and experimental data are not directly accessible.
• Column-level documentation for any underlying data tables is absent; field semantics must be inferred from the text.
• Row count for any potential data is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Combined experimental study involving animal challenge, microbiota analysis via 16S rRNA sequencing, and computational network pharmacology.

Freshness

Last updated 2026-05-05 04:13:27