Galectin-9 Role in Glioma Microglia Adhesion and Phagocytosis

Eighteen isocitrate dehydrogenase-stratified glioma patient samples form the basis for single-cell transcriptomics data identifying LGALS9 as a key mediator in IDH-wild type gliomas. The research data includes spectral cytometry, immunohistochemistry, and Western blotting analyses confirming Galectin-9 expression in glioma-associated microglia. Functional validation was performed using an ex-vivo co-culture system of primary human microglia and patient-derived glioma stem cells.

Use Cases

• Analyze the ligand-receptor interactome data from dendritic cells and microglia to lymphoid cells to identify key mediators like LGALS9.
• Validate Galectin-9 expression patterns in glioma-associated microglia using the spectral cytometry and immunohistochemistry data.
• Model cell adhesion and phagocytosis pathways using transcript data for coronin 1A, integrin, FcγR, and Rab family proteins from Galectin-9+ microglia.
• Simulate the impact of Galectin-9 knockdown on glioma stem cell adhesion and phagocytosis using data from the siRNA and antibody neutralization experiments.

Strengths

• Data is derived from tumor samples of eighteen isocitrate dehydrogenase-stratified glioma patients.
• Findings are validated through multiple methods: spectral cytometry, immunohistochemistry, and Western blotting.
• Functional role is tested in an ex-vivo co-culture system using primary human microglia and patient-derived glioma stem cells.

Limitations

• Sample size is limited to eighteen patient brains, which may restrict statistical power for some subgroup analyses.
• The primary data file is a 4.9 MB DOCX document, which is not a standard structured data format for computational analysis.
• The specific row and column structure of the underlying transcriptomic or cytometry data is not provided in the input.

Provenance

Source

Pravesh Gupta, published on figshare.

Collection Method

Single-cell transcriptomics of tumor-associated CD45+ leukocytes, followed by spectral cytometry, immunohistochemistry, Western blotting, and functional co-culture assays.

Freshness

Last updated March 24, 2026.