Human Milk Extracellular Vesicle Isolation Protocol for Preterm Infant Nutrition

This dataset describes a scaled-up protocol for isolating extracellular vesicles (HMEVs) from human milk, increasing processed volume from 25 mL to 63 mL and reducing isolation time from 14 hours to 7.7 hours. The method achieved high purity, with up to 3.98 × 10^13 particles per gram of protein, and the final product was stabilized in a 5% glucose solution for up to 30 days at -80°C.

Use Cases

• Analyze the relationship between ultracentrifugation speed modifications and the approximate 2-fold increase in EV recovery reported in the results.
• Model the impact of omitting filtration on HMEV yield, given the reported 4.3-fold increase in recovery.
• Evaluate the stability of HMEVs resuspended in 5% glucose solution based on the reported preservation data for up to 30 days at -80°C.
• Assess protocol scalability by comparing the initial 25 mL laboratory-scale process to the final 63 mL clinically compatible volume.

Strengths

• Protocol details specific quantitative outcomes, including a 2-fold EV recovery increase and a 4.3-fold recovery increase from process modifications.
• Reports high purity metrics, specifically up to 3.98 × 10^13 particles/g protein for the isolated HMEVs.
• Describes a finalized, sterile process with negative endotoxin testing, suitable for clinical applications.

Limitations

• The dataset is a 1.2 MB PDF document describing a method, not a structured tabular dataset with rows and columns for direct computational analysis.
• Sample data and specific columnar measurements (e.g., per-donor EV counts, miRNA profiles) are unavailable within the provided file.
• The temporal and geographic scope of the source human milk samples is not specified.

Provenance

Source

figshare, authored by Jose Luis Moreno-Casillas.

Collection Method

Laboratory-scale protocol development and scaling for isolating extracellular vesicles from human milk.

Freshness

Last updated 2026-03-20.