Isoviolanthin Effects on IL-1β-Stimulated Rat Chondrocytes: In Vitro Assay Data

Experimental data from a study investigating the anti-inflammatory effects of isoviolanthin on interleukin-1β-stimulated rat chondrocytes. The dataset includes results from network pharmacology, molecular docking, and in vitro assays measuring gene expression, protein levels, and cell viability. The data was authored by Jian-Li Yin and last updated on 2026-05-22.

Use Cases

• Validate network pharmacology predictions for drug-target interactions based on the 141 overlapping targets identified.
• Analyze the inhibitory effects of isoviolanthin on PI3K-AKT and MAPK signaling pathways based on Western blot and immunofluorescence data.
• Correlate changes in inflammatory mediator expression (e.g., IL-6, TNF-α, COX2) with extracellular matrix metabolism markers (e.g., collagen II, ACAN) from RT-qPCR results.
• Assess the concentration-dependent effects of isoviolanthin on cell viability and matrix-degrading enzyme (MMP) expression mentioned in the description.

Strengths

• Data is derived from multiple experimental methods including network pharmacology, molecular docking, Western blot, RT-qPCR, and immunofluorescence.
• Specific working concentration (25 μM) and time point (24 h) for isoviolanthin treatment are defined.
• The dataset is licensed under CC-BY-4.0, permitting open sharing and adaptation.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is from a single in vitro model using rat chondrocytes; translational relevance to human cells requires further validation as noted in the conclusion.

Provenance

Source

figshare

Collection Method

Generated through in vitro laboratory assays and computational analysis (network pharmacology, molecular docking) as part of a research study.

Freshness

Last updated 2026-05-22 11:16:24; freshness should be verified.