Mouse and Human Cortex Cellular Composition from 3D Confocal Counting

A dataset quantifying the proportions of neurons, glia, and vascular cells across cortical layers in the mouse primary somatosensory cortex and human temporal cortex. Over 25,000 cells in the mouse and 13,000 cells in the human cortex were individually identified and classified using immunocytochemistry and a direct 3D counting method with EspINA software. The data was authored by Sergio Plaza-Alonso and last updated on 2026-04-23.

Use Cases

• Compare species-specific cellular proportions based on the reported neuron, glia, and vascular cell counts.
• Analyze laminar organization of the cortex based on the full-thickness layer (I-VI) characterization.
• Calculate glia-to-neuron and non-neuron-to-neuron ratios for specific cortical regions (mouse S1HL and human BA21).
• Validate stereological or tissue-homogenization techniques against the direct 3D counting method described.

Strengths

• Includes counts for over 25,000 cells in mouse and 13,000 cells in human cortex.
• Provides specific cellular proportion metrics, such as neurons representing ~60% of cells in mouse and ~30% in human.
• Reports calculated ratios: glia-to-neuron ratio of 0.4 (mouse) and 1.5 (human), and non-neuron-to-neuron ratio of 0.6 (mouse) and 2.3 (human).
• Uses a direct 3D confocal counting method that preserves laminar and spatial context.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small (51.5 KB), indicating limited scope, likely a summary document rather than raw cell data.

Provenance

Source

figshare, author Sergio Plaza-Alonso

Collection Method

Immunocytochemical techniques and direct 3D counting using EspINA software.

Freshness

Last updated 2026-04-23 08:34:35; freshness should be verified.