Lyar Knockout Mouse ESC Data on Proliferation and Differentiation

Encompassing molecular biology data from a study investigating the role of the Lyar gene in mouse embryonic stem cells. The research used CRISPR/Cas9 to generate Lyar knockout lines and measured effects on proliferation, apoptosis, cell cycle, and expression of germ layer markers. The data is presented in a PDF file of 809.7 KB.

Use Cases

• Analyze the relationship between Lyar knockout status and measured outcomes like proliferation rate and apoptosis levels.
• Compare expression levels of germ layer-specific markers (e.g., Gsc, T, Gata4, Sox17, Pax6) between wild-type and Lyar-deficient mESCs.
• Investigate the correlation between p53 and p21 protein expression changes and cell cycle progression defects in the knockout clones.
• Model the impact of Lyar deficiency on multi-lineage differentiation potential using data from the embryoid body formation assay.

Strengths

• Data is derived from a controlled experimental study using CRISPR/Cas9 gene editing in mouse embryonic stem cells.
• The study investigates multiple biological processes: proliferation, apoptosis, cell cycle, and differentiation across three germ layers.
• The dataset is shared under a permissive CC BY 4.0 license, facilitating reuse.

Limitations

• The core data is embedded within a PDF document (809.7 KB), which is not a structured, machine-readable format like CSV.
• The sample size (number of biological replicates, cell lines, or data points) is not specified in the provided description.
• The data is static and represents a single experimental study, not a time-series or longitudinal collection.

Provenance

Source

figshare

Collection Method

Experimental data generated via CRISPR/Cas9 knockout in mouse embryonic stem cells, followed by assays for proliferation, apoptosis, cell cycle, and differentiation marker expression.

Freshness

The record was last updated on 2026-03-20.