Metabolomic Profiling of Lipid Reprogramming During 3T3-L1 Adipocyte Differentiation

An 8-day time-course metabolomic profile of murine 3T3-L1 cells differentiating into adipocytes, collected by Nanping Shi. The dataset identifies 170 differential metabolites between pre-differentiation and 4-day stages, and 246 between pre-differentiation and mature stages, with pronounced changes in triglycerides and phosphatidylcholines. Data was last updated on 2026-05-26.

Use Cases

• Modeling lipid metabolism dynamics during adipogenesis based on the 170 identified differential metabolites.
• Investigating glycerolipid and glycerophospholipid pathway activity mentioned in the KEGG enrichment results.
• Training classifiers to predict adipocyte differentiation stage based on metabolite profiles from the Pre, Middle, and Mature time points.
• Analyzing correlations between lipid and amino acid metabolism networks highlighted in the study.

Strengths

• Identifies 170 differential metabolites between Pre and Middle stages and 246 between Pre and Mature stages.
• Provides data across three distinct time points (Pre, 4-day Middle, 8-day Mature) during differentiation.
• Includes results from KEGG pathway enrichment analysis linking metabolites to specific metabolic pathways.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (69.4 KB), indicating limited scope, likely containing summary results rather than raw measurements.

Provenance

Source

Nanping Shi via figshare

Collection Method

Metabolites extracted from cultured 3T3-L1 cells and analyzed by ultra-performance liquid chromatography coupled with mass spectrometry.

Time Range

Covers an 8-day differentiation period.

Freshness

Last updated 2026-05-26 05:52:38; freshness should be verified.