Methodology for a High-Throughput 3D Tuberculoma Bioplatform

Presenting a PDF document describing a methodology for generating a high-throughput, 3D cell culture model of tuberculomas for tuberculosis research. The model is designed in a standard 96-well plate format, enabling scalable screening of therapeutics. It was authored by Suraj B. Sable and is available under a CC BY 4.0 license.

Use Cases

• Implement the described 'mycobacteria-in-spheroid' co-culture workflow to generate 3D tuberculoma-like structures for host-pathogen interaction studies.
• Apply the high-throughput screening (HTS)-compatible bioplatform to test chemical and genetic libraries for drug efficacy against tuberculosis.
• Utilize the cryo-preservation methodology outlined to create shelf-stable, reproducible models for commercial discovery programs in granulomatous diseases.
• Employ the platform's serial quantitation capability to monitor lesion resolution and drug effects over days to weeks following a single treatment.

Strengths

• The methodology is explicitly designed for high-throughput screening (HTS) compatibility in a standard 96-well plate format.
• The bioplatform is described as having ease of generation, pliability, cryo-shelf stability, and reproducibility.
• The document is recent, with a last update date of March 20, 2026.

Limitations

• The dataset is a 211.4 KB PDF document, not a structured data table, limiting direct computational analysis.
• No raw experimental data, column definitions, or sample results are provided within the described file.
• The methodology's performance and reproducibility are dependent on the user's laboratory implementation.

Provenance

Source

figshare, authored by Suraj B. Sable.

Collection Method

A described laboratory methodology for generating 3D tuberculoma-like cell culture models.

Freshness

Last updated 2026-03-20.