Methods for Detecting H5N1 Virus in Dairy Processing Environments

A study evaluating molecular methods for detecting influenza A virus in milk and on dairy farm surfaces. The research optimized an aluminum chloride precipitation method for virus concentration and compared swabbing techniques. Results include detection limits for H5N1 and H3N2 in raw and pasteurized milk, and virus recovery rates from surfaces like stainless steel and silicone.

Use Cases

• Optimize virus concentration protocols based on the described aluminum chloride precipitation method for milk.
• Compare surface sampling techniques based on the evaluation of ISO 15216:1-based swabbing versus sponge-based methods.
• Assess capsid integrity assays based on the use of propidium monoazide and platinum (IV) chloride for distinguishing infectious virus.
• Establish detection thresholds for H5N1 monitoring based on the reported genome copy limits per liter in milk.

Strengths

• Provides specific detection limits (LoD95%) for H5N1 and H3N2 viruses in raw and pasteurized milk.
• Reports mean virus recovery percentages (e.g., 22.46% ± 11.86% in pasteurized milk) from experimental methods.
• Compares surface sampling methods on specific materials like stainless steel and silicone.

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is a 706.5 KB DOCX file, suggesting limited scope and likely being a methods document rather than a primary data table.

Provenance

Source

Pablo Puchades-Colera

Collection Method

Laboratory study evaluating molecular detection methods.

Freshness

Last updated 2026-06-04 05:48:07; freshness should be verified.