MFGE8-primed Fibroblasts: RNA-seq Data for Diabetic Wound Healing Research

A 2.4 MB dataset published on figshare in 2026 by Ling Pan. It contains RNA sequencing data from human diabetic foot ulcers (DFUs) and db/db mouse wounds, used to investigate the role of MFGE8 in fibroblast-driven immune responses. The data supports findings on MFGE8 downregulation and its therapeutic potential in chronic wound healing.

Use Cases

• Identify gene expression alterations in diabetic wound fibroblasts based on integrated bulk and single-cell RNA-seq data mentioned in the description
• Validate molecular regulators of fibroblast immune activity using cytokine screening and migration assay results described
• Assess therapeutic potential of MFGE8-prestimulated fibroblasts for accelerating wound closure based on in vivo mouse model data

Strengths

• Data is derived from integrated human (GSE199939, GSE165816) and mouse (db/db) RNA-seq datasets, providing cross-species validation
• Includes results from multiple validation methods: immunofluorescence, RT-qPCR, Western blot, ELISA, and Transwell migration assays
• Published under a permissive CC-BY-4.0 license, allowing for reuse and redistribution

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment for large-scale analyses
• The dataset is a 2.4 MB PDF, which may contain processed results rather than raw sequencing data

Provenance

Source

figshare

Collection Method

Integrated analysis of bulk RNA-seq (GSE199939) and single-cell RNA-seq (GSE165816) from human DFUs, validated with db/db mouse wound models.

Time Range

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Freshness

Last updated 2026-05-06 07:24:41; freshness should be verified

Geography

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