QobuR Model: Microbial Effects on Corneal Proteoglycan Gene Expression

A three-dimensional in vitro corneal epithelium model, QobuR, was co-cultured with individual bacterial species and a mixed microbial consortium. Transcriptional profiles of genes involved in proteoglycan and glycosaminoglycan biosynthesis were analyzed via qRT-PCR and immunohistochemistry. The dataset, authored by Noelia Blanco-Agudín and shared under CC-BY-4.0, is a 193.0 KB PDF file.

Use Cases

• Analyzing transcriptional responses of corneal epithelial cells to specific bacterial exposure based on qRT-PCR data.
• Comparing gene expression patterns for enzymes involved in heparan sulfate and chondroitin sulfate sulfation.
• Evaluating the effect of a mixed microbial consortium versus individual species on epithelial model physiology.
• Assessing the similarity between microbiota-exposed in vitro models and donor-derived corneal epithelium.

Strengths

• Data is derived from a controlled in vitro model designed to reproduce complex cellular interactions.
• Analysis includes both qRT-PCR and immunohistochemical validation of transcriptional changes.
• The dataset is openly shared under a CC-BY-4.0 license.

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Transcriptional analysis via qRT-PCR and immunohistochemistry following co-culture experiments.

Freshness

Last updated 2026-05-28 04:50:32; freshness should be verified.