miR-142-5p miRNA Expression Profiles in T-Cell Subsets

Hongqiong Wang uploaded a 2.2 MB document describing miRNA expression profiles in T-cell subsets via figshare on 2026-05-18. The study investigates hsa-miR-142-5p expression across naïve, stem cell-like memory, central memory, and effector memory T cells. It details functional validation of miR-142-5p targeting PRKCB and its effects on T-cell differentiation markers, proliferation, and cytokine secretion.

Use Cases

• Analyze miRNA expression patterns across T-cell differentiation stages based on high-throughput sequencing profiles mentioned in the description
• Validate gene targets for specific miRNAs in T-cell biology based on dual-luciferase reporter assay results described
• Model the relationship between miRNA expression and T-cell subset proportions based on measured changes in TN, TSCM, and TEM/TEFF cells

Strengths

• The document provides detailed methodological and results sections for a specific immunology study
• It includes validation of a direct miRNA target (PRKCB) via dual-luciferase reporter assays
• Functional outcomes are described across multiple metrics including gene expression, surface markers, cell proportions, proliferation, apoptosis, and cytokine secretion

Limitations

• The dataset is a 2.2 MB DOCX file, likely containing a research paper or data sheet rather than raw data tables
• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment

Provenance

Source

figshare

Collection Method

High-throughput sequencing of miRNA expression profiles from isolated T-cell subsets, followed by bioinformatics analysis and functional validation.

Freshness

Last updated 2026-05-18 05:36:46; freshness should be verified