Data Sheet 1_Multi-omics characterization of β-myrcene-evolved Pseudomonas sp. M1 reveals

Two evolved Pseudomonas sp. M1 isolates, M2C19 and M3C22, were characterized after 600 generations of adaptive laboratory evolution under β-myrcene selection. Filipa Soares published this dataset on figshare in April 2026, containing comparative genomics, quantitative proteomics, and metabolite profiling data. The data reveals convergent FleQ mutations, altered catabolic efficiency, and distinct proteometabolic strategies for improved monoterpene processing.

Use Cases

• Analyze convergent genetic mutations based on comparative genomics data mentioned in the description
• Model metabolic pathway flux based on metabolite profiling showing 10.6-fold myrcenoic acid accumulation
• Study temporal protein expression dynamics based on proteome profiling of genomic island induction
• Investigate trade-offs between motility and catabolism based on proteomic data showing reduced chemotaxis systems
• Identify genetic targets for strain improvement based on the adaptive laboratory evolution results

Strengths

• Includes multi-omics data types: comparative genomics, quantitative proteomics, and metabolite profiling
• Documents a 33% higher final OD600 in evolved strains compared to wild-type
• Shows specific metabolite changes, including a 10.6-fold increase in myrcenoic acid accumulation
• Based on 600 generations of adaptive laboratory evolution, providing a robust evolutionary context

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Data is contained in a single 3.1 MB XLSX file, which may limit the depth of individual omics datasets

Provenance

Source

figshare

Collection Method

Adaptive laboratory evolution, comparative genomics, quantitative proteomics, and metabolite profiling

Time Range

Laboratory study timeframe not specified

Freshness

Last updated 2026-04-13 04:35:49

Geography

Laboratory study location not specified