QI9/A24 T-Cell Repertoire Analysis for SARS-CoV-2 Spike Protein Epitope

219 clonotypes and 169 αβ TCR pairs were isolated from six donors to characterize the T-cell response to a conserved SARS-CoV-2 spike protein epitope. The dataset, authored by Yoshiki Aritsu and last updated on 2026-04-27, includes analysis of peptide modifications and their impact on T-cell recognition and vaccine efficacy. It is shared under a CC-BY-4.0 license on figshare as a 1.5 MB PDF document.

Use Cases

• Analyzing TCR repertoire diversity based on the 219 identified clonotypes and 169 αβ TCR pairs.
• Evaluating T-cell recognition sensitivity based on comparisons between wild-type and N-terminally modified peptides.
• Investigating epitope modification strategies for vaccine enhancement based on the pyroglutamylated peptide's increased protease resistance and immunogenicity.
• Studying cross-reactivity and substitution tolerance based on the analysis of alanine-substituted and homologous coronavirus-derived peptides.

Strengths

• Includes specific quantitative results: 219 clonotypes and 169 αβ TCR pairs identified from six donors.
• Compares wild-type and modified peptide performance in both human samples and HLA-A24 transgenic mice.
• Provides detailed immunological analysis of a conserved, immunodominant epitope (QYIKWPWYI; residues 1208–1216).

Limitations

• Dataset is presented as a single 1.5 MB PDF; underlying raw data tables or sequences are not directly accessible.
• Row count and column-level documentation are unknown, limiting suitability assessment for computational reuse.
• Data may reflect bias inherent to the specific HLA-A*24:02-restricted epitope and the six studied donors.

Provenance

Source

figshare, author Yoshiki Aritsu.

Collection Method

Likely contains experimental results from TCR repertoire analysis, peptide synthesis, and immunization studies.

Freshness

Last updated 2026-04-27 05:16:30; freshness should be verified.