Platycladus orientalis Fraction Effects on Retinal Müller Cells Under Glucose Stress

A 14.9 MB document authored by Jing Xian Lim details the neuroprotective effects of a plant fraction on retinal Müller cells. The study identifies diterpenoids, monoterpene esters, phenolic glycosides, and saturated fatty acids in the fraction and reports an IC50 cytotoxicity value of 0.32 mg/mL. It was uploaded to figshare on 2026-05-22.

Use Cases

• Analyze the neuroprotective mechanisms of plant fractions based on the modulation of AKT1/mTOR and Raf-1/MEK1/2 pathways described.
• Investigate cytotoxicity thresholds for potential therapeutics based on the reported IC50 value.
• Study the relationship between AMPK activation and VEGF/VEGFR2 signaling inhibition based on the described experimental results.
• Identify bioactive compounds for further research based on the phytochemical analysis listing diterpenoids, monoterpene esters, phenolic glycosides, and saturated fatty acids.

Strengths

• The document provides a specific cytotoxicity measurement (IC50 = 0.32 mg/mL).
• The phytochemical analysis lists concrete compound classes: diterpenoids, monoterpene esters, phenolic glycosides, and saturated fatty acids.
• The mechanistic study details specific protein and gene biomarkers (VEGF, VEGFR2, mTOR, Raf-1, MEK1/2, AKT1).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The data is contained in a single 14.9 MB DOCX file, which may require parsing to extract structured information.

Provenance

Source

figshare

Collection Method

In vitro cell culture experiments and liquid chromatography-mass spectrometry analysis.

Freshness

Last updated 2026-05-22 04:27:59; freshness should be verified.