Pediatric Liver Transplant Immunosuppressant Withdrawal Plasma Proteomics Study

A 2026 single-center cohort study analyzed baseline plasma proteomics and HDAC1 levels from pediatric liver transplant recipients undergoing immunosuppressant withdrawal. The primary analytic cohort included 31 recipients, with plasma proteomics from 10 patients and HDAC1 ELISA validation in 39 recipients. The study identified neutrophil-associated pathways and HDAC1 as a candidate biomarker for immune tolerance outcomes.

Use Cases

• Analyze baseline plasma proteomic signatures from 10 patients to identify neutrophil-related pathways associated with immune tolerance outcomes.
• Validate HDAC1 ELISA quantification results from 39 recipients for baseline risk stratification of immunosuppressant withdrawal.
• Correlate immunohistochemistry HDAC1 staining results from 10 baseline liver biopsies with plasma proteomic and ELISA findings.
• Evaluate the diagnostic performance of plasma HDAC1 levels using ROC analysis to distinguish immune-tolerant from non-immune-tolerant outcomes.

Strengths

• Includes validation across multiple assay types: LC-MS proteomics, ELISA, and immunohistochemistry.
• Primary analytic cohort of 31 pediatric liver transplant recipients undergoing planned immunosuppressant withdrawal.
• HDAC1 validation performed on plasma from 39 recipients, demonstrating moderate discriminative performance (AUC=0.81).

Limitations

• Small sample sizes for key analyses: proteomics from 10 patients and immunohistochemistry from 10 biopsies.
• Single-center study design limits generalizability of findings.
• Data is contained in a 253.4 KB DOCX file, not a structured data table, limiting direct computational analysis.

Provenance

Source

Ruofan Wang via figshare.

Collection Method

Plasma analyzed via liquid chromatography-mass spectrometry (LC-MS) proteomics and HDAC1 ELISA; liver biopsies assessed by immunohistochemistry.

Time Range

Study follow-up cut-off was June 30, III.

Freshness

Last updated March 26, III.

Geography

Single-center cohort study; specific location unknown.