NMN Protects Vascular Endothelial Cells from M1 Macrophage-Induced Hyperpermeability

526.1 KB PDF authored by Takeshi Katayoshi, last updated 2026-05-18, describes an in vitro mechanistic study. The research examines how Nicotinamide Mononucleotide (NMN) protects endothelial barrier integrity against inflammatory stimuli from M1 macrophages, using a three-dimensional co-culture model of HUVECs and M1 macrophages.

Use Cases

• Analyze NMN's protective effect on endothelial hyperpermeability based on fluorescent dextran/LDL passage measurements
• Investigate the role of the IL-1β and NF-κB pathway in VE-cadherin degradation based on mechanistic analysis
• Study the NAD+-SIRT1 axis in endothelial barrier protection based on inhibitor and siRNA knockdown experiments
• Compare the effects of different NAD+ precursors like nicotinamide riboside based on the described experimental results

Strengths

• The study provides a detailed mechanistic analysis of NMN's effect on endothelial barrier integrity.
• The experimental model is described as a three-dimensional co-culture of HUVECs and M1 macrophages.
• The dataset is openly licensed under CC-BY-4.0.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The data is a single 526.1 KB PDF file, representing a limited scope.

Provenance

Source

figshare

Collection Method

In vitro laboratory study using a three-dimensional co-culture model.

Freshness

Last updated 2026-05-18 04:10:34; freshness should be verified.