RNA Sequencing Data for a Novel MYH11::GLI3 Fusion in Ileal Leiomyoma

A 1.5 MB PDF document authored by Ioannis Panagopoulos and last updated on 2026-04-13 details the identification of a novel gene fusion in a gastrointestinal tumor. The study used RNA sequencing with multiple fusion-detection algorithms on an ileal leiomyoma, validated by RT-PCR and Sanger sequencing. It reports the first MYH11::GLI3 chimera in this context, expanding the molecular spectrum of these neoplasms.

Use Cases

• Study the molecular spectrum of gastrointestinal leiomyomas based on the reported novel gene fusion.
• Investigate alternative Hedgehog pathway perturbation mechanisms based on the deregulation of GLI3 mentioned.
• Benchmark fusion-detection algorithms using the described RNA sequencing and validation methodology.
• Explore the biological relevance of smooth muscle-specific MYH11 expression in conjunction with GLI3-mediated signaling.

Strengths

• Describes a novel, first-reported MYH11::GLI3 gene fusion validated by RT-PCR and Sanger sequencing.
• Released under a permissive CC-BY-4.0 license, allowing for reuse and redistribution.
• The underlying RNA sequencing data was analyzed with multiple fusion-detection algorithms for robustness.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is a 1.5 MB PDF; the underlying raw sequencing data is not directly provided.
• The frequency and clinical significance of GLI3-rearranged tumors remain to be determined, as noted in the conclusion.

Provenance

Source

Ioannis Panagopoulos via figshare.

Collection Method

RNA sequencing performed on an ileal leiomyoma, with validation by RT-PCR and Sanger sequencing.

Time Range

The study period is not specified.

Freshness

Last updated 2026-04-13 05:01:12; freshness should be verified.

Geography

Geographic coverage is not specified.