Tetradium Daniellii Callus Culture Data for Alkaloid Optimization

Experimental data from a study optimizing callus culture conditions for the medicinal plant Tetradium daniellii. The dataset includes results from culturing leaf and petiole tissues on four media types under light and dark conditions, with alkaloid content quantified via UHPLC-MS/MS. It was authored by Ji Won Kim and last updated in May 2026.

Use Cases

• Compare alkaloid accumulation efficiency across different plant tissue culture media based on the described MS, WPM, DKW, and SH media.
• Analyze the effect of light versus dark incubation on callus induction and secondary metabolite production based on the described experimental conditions.
• Benchmark callus-derived alkaloid yields against traditional plant tissues like leaves and immature fruits based on the quantitative comparisons described.
• Identify optimal culture conditions for maximizing specific bioactive compounds like rutaecarpine and evodiamine based on the reported quantitative results.

Strengths

• Quantitative results include specific alkaloid concentrations, such as rutaecarpine reaching 140.83 mg·kg⁻¹ in callus.
• Experimental design is clearly described, comparing four culture media and two light conditions.
• Data is shared under a permissive CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect methodological bias inherent to the specific laboratory protocols used.

Provenance

Source

figshare

Collection Method

Laboratory experiment involving in vitro plant tissue culture and UHPLC-MS/MS analysis.

Freshness

Last updated 2026-05-13 05:51:52; freshness should be verified.

Geography

Plant species is native to Korea and China; specific study location is unknown.