Mouse Lung Single-Cell RNA Sequencing Data on Ozone-Induced Inflammation

Single-cell RNA sequencing data from lung tissue of male BALB/c mice exposed to ozone or ambient air. The dataset likely contains gene expression profiles used to identify osteopontin-producing cells and infer cell-cell communication. It was uploaded by Hiroki Tashiro to figshare in March 2026.

Use Cases

• Identify osteopontin-producing cell types based on single-cell RNA sequencing data mentioned in the description
• Analyze DC-neutrophil interactions inferred from CellChat analysis
• Validate gene expression changes in neutrophils stimulated with osteopontin in vitro
• Correlate osteopontin levels with neutrophilic inflammation and airway hyperresponsiveness metrics

Strengths

• Includes functional validation data from clodronate liposome and neutralizing antibody treatments
• Combines in vivo ozone exposure experiments with in vitro neutrophil stimulation assays
• License is CC-BY-4.0, allowing for broad reuse

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Data is from a single mouse strain (BALB/c) and sex (male), limiting generalizability

Provenance

Source

figshare

Collection Method

Experimental data from mouse exposure studies, single-cell RNA sequencing, and in vitro neutrophil stimulation.

Freshness

Last updated 2026-03-19 09:08:50; freshness should be verified