Data Sheet 1: Gene Expression Data for Heat Stress Resistance in Cotton

119.5 KB PDF details a genetic transformation study on cotton. The research identified and overexpressed the FTSH gene GH_DO1G0225.1, resulting in transgenic plants with approximately 5.5-fold higher gene expression across three generations. Shahid Iqbal authored this dataset, last updated on 2026-05-15.

Use Cases

• Validate gene expression levels for heat tolerance based on quantitative real-time PCR results.
• Compare transformation effectiveness and seed germination rates between transgenic and non-transgenic cotton lines.
• Study the stability of transgene expression across T0, T1, and T2 plant generations.
• Identify candidate FTSH family genes for functional validation in other crops.

Strengths

• Dataset includes specific quantitative results, such as a 1.89% transformation effectiveness and a 71.42% seed germination index.
• Gene expression fold-changes (e.g., 5.59-fold increase) are reported for multiple plant generations.
• The research methodology, including Agrobacterium-mediated transformation and PCR validation, is clearly described.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 119.5 KB size suggests the dataset is a small, focused document rather than a large-scale data repository.

Provenance

Source

figshare

Collection Method

Genetic transformation and quantitative real-time PCR analysis.

Freshness

Last updated 2026-05-15 04:29:01; freshness should be verified.