Pasteurized Akkermansia muciniphila Effects on Bone Loss in Obese Mice

A 2.8 MB PDF research article authored by Si Chen details a study on pasteurized Akkermansia muciniphila alleviating bone loss in high-fat diet-induced obese mice. The work uses fecal microbiota transplantation, micro-CT, histomorphometry, flow cytometry, and RNA sequencing to investigate mechanisms involving Treg differentiation and the Nr4a1 gene. The dataset was last updated on May 22, 2026 and is available under a CC-BY-4.0 license.

Use Cases

• Analyze the relationship between gut microbiota dysbiosis and bone loss based on the described mouse model and fecal transplantation.
• Investigate the differential effects of live versus pasteurized Akkermansia muciniphila on bone homeostasis based on the experimental results.
• Study the role of regulatory T (Treg) cell differentiation in inhibiting osteoclastogenesis based on the flow cytometry and immunofluorescence data.
• Validate the Nr4a1-dependent mechanism linking probiotic intervention to bone protection based on the transcriptomic and knockout mouse experiments.

Strengths

• The dataset is a detailed research article with a specific mechanistic focus on the Nr4a1/Treg axis.
• It includes results from multiple experimental techniques: micro-CT, histomorphometry, ELISA, flow cytometry, and RNA sequencing.
• The data is openly available under a CC-BY-4.0 license.

Limitations

• The dataset is a PDF document; the underlying raw experimental data (e.g., column-level measurements) is not directly accessible.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.
• The study is based on a mouse model; applicability to human physiology requires further investigation.

Provenance

Source

figshare

Collection Method

Experimental research involving high-fat diet-induced obese mice, probiotic supplementation, and various laboratory analyses.

Freshness

Last updated 2026-05-22 04:30:05; freshness should be verified.