Phenotypic Transformation of HCC827 Cells Under Human Platelet Lysate Culture

A 19.4 MB dataset on figshare documents an investigation into human platelet lysate (HPL) as a replacement for fetal bovine serum (FBS) in culturing non-small cell lung carcinoma cells. The work, authored by Clemens Woitaske-Proske and last updated in May 2026, includes ELISA data showing TGF-β1 levels and kinome activity profiling results for HCC827, A549, and H1299 cell lines. It highlights distinct phenotypic changes in HCC827 cells when cultured with HPL.

Use Cases

• Compare cytokine content (FGF2, TGF-β1) between serum supplements based on ELISA data mentioned in the description
• Analyze cell-type-specific signaling pathway alterations based on kinome activity profiling data for HCC827, A549, and H1299 cells
• Investigate phenotypic changes (spheroid morphology, f-actin structures) in cancer cell lines under different culture conditions
• Evaluate hybrid epithelial-to-mesenchymal transition (EMT) states using marker data (Vimentin, SNAI1) from Western blot analysis

Strengths

• Includes comparative data on FGF2 and TGF-β1 levels, noting a 2-fold higher amount of TGF-β1 in HPL compared to FBS
• Provides kinome activity profiling data across multiple time points for three distinct NSCLC cell lines (HCC827, A549, H1299)
• Dataset is openly available under a CC-BY-4.0 license

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Data is contained within a 19.4 MB DOCX file, which may require extraction and formatting for analysis

Provenance

Source

Clemens Woitaske-Proske

Collection Method

Experimental cell culture, ELISA, Western blot, and kinome activity profiling.

Freshness

Last updated 2026-05-13 05:48:34