Data Sheet 1: Phosphatase PTP-4 Downregulates Host Defense in C. elegans

Data Sheet 1 contains results from a study on phosphatase regulation of innate immunity in Caenorhabditis elegans. The dataset, authored by Jing Xie and last updated on 2026-06-01, details findings from a genome-wide RNAi screen of 166 phosphatase genes and a small-molecule screen identifying baicalin as an inhibitor of P. aeruginosa infection.

Use Cases

• Validate phosphatase gene candidates from the RNAi screen based on host survival data.
• Analyze protein-protein interaction data between PTP-4 and ATLN-1 mentioned in the description.
• Model the dose-response relationship of baicalin based on the reported binding affinity (KD = 28.69 μM).
• Investigate the hyperactivation of the p38 MAPK (PMK-1) pathway in ptp-4 deficient strains.
• Screen for other small-molecule inhibitors based on the structural and biochemical analysis methods described.

Strengths

• Dataset is derived from a genome-wide RNAi screen covering 166 phosphatase genes.
• Includes validation data from multiple experimental methods, including yeast two-hybrid screening, Co-IP, and mutagenesis assays.
• Small-molecule screen identified a specific compound (baicalin) with a measured binding affinity (KD = 28.69 μM).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (17.2 KB), indicating limited scope.

Provenance

Source

Jing Xie via figshare

Collection Method

Genome-wide RNAi screen, yeast two-hybrid screening, Co-IP, mutagenesis, and small-molecule screening.

Freshness

Last updated 2026-06-01 04:21:46; freshness should be verified.