RamAp Plasmid Regulator: Salmonella Gene Expression and Virulence Data

A research article investigating the plasmid-borne transcriptional regulator RamAp in Salmonella enterica serovar Typhimurium LT2. The study, authored by Yu-Ping Hong and last updated in May 2026, includes data from promoter activity assays, electrophoretic mobility shift assays (EMSAs), and functional assays on motility and virulence. The findings demonstrate how RamAp reprograms host gene expression to integrate antibiotic resistance, stress resilience, and virulence-associated traits.

Use Cases

• Study plasmid-mediated gene regulation based on the investigation of the RamAp homolog.
• Analyze the role of truncated ISEcp1 elements as transcriptional enhancers based on promoter activity assays.
• Model bacterial stress adaptation pathways based on targets like sodA and micF identified in the study.
• Investigate the functional overlap between plasmid-borne and chromosomal regulators based on the comparison of RamAp and RamA.

Strengths

• Includes data from multiple experimental methods: GFP reporter constructs, EMSAs, and functional assays.
• Focuses on specific, conserved regulatory targets: micF, sodA, flhDC, and acrAB.
• Published under a permissive CC-BY-4.0 license for reuse.

Limitations

• The primary data format is a PDF (3.0 MB), which may require extraction of underlying data.
• Row count and column-level documentation are unknown, limiting suitability assessment.
• Data may reflect experimental bias inherent to the specific Salmonella strain and laboratory conditions used.

Provenance

Source

figshare, author Yu-Ping Hong

Collection Method

Experimental laboratory study using Salmonella enterica serovar Typhimurium LT2.

Freshness

Last updated 2026-05-28 05:58:19