Flavobacterium Columnare Gene Knockout Effects on Membrane and Virulence

A 3.8-fold increase in outer membrane vesicle production was observed in a Flavobacterium columnare sppA gene knockout mutant. Ruoxi Zhu published this dataset on figshare in May 2026, detailing pleiotropic effects including loss of gliding motility and attenuated virulence. Transcriptomic profiling revealed upregulated genes in membrane stress response and efflux systems.

Use Cases

• Analyzing the link between signal peptide peptidase function and outer membrane vesicle production based on the 3.8-fold OVM increase
• Studying the impact of gene deletion on bacterial motility based on the loss of gliding motility phenotype
• Investigating virulence attenuation in bacterial infections based on the 20% higher fish survival rate
• Examining transcriptomic responses to membrane stress based on upregulated efflux pump and stress response genes

Strengths

• Includes specific quantitative findings like a 3.8-fold increase in OMV production and a 20% higher fish survival rate
• Focuses on a targeted gene knockout study with clear experimental methodology described
• Released under a CC-BY-4.0 license, facilitating open reuse

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• The dataset is a single 1.5 MB DOCX file; data structure and scale are limited

Provenance

Source

figshare

Collection Method

Targeted in-frame gene knockout approach and transcriptomic profiling in Flavobacterium columnare

Freshness

Last updated 2026-05-22 04:28:01; freshness should be verified