Protease-Activated Receptor 1 Signaling Data for G Protein Selectivity Studies

A 5.4 MB dataset by Braden S. Fallon, last updated in May 2026, investigates protease-activated receptor 1 (PAR1) as a model for G protein signaling selectivity. The data integrates results from transducer-wide biosensor assays, transcriptional response element analysis, and studies on primary human platelets. It compares signaling pathways activated by thrombin versus activated protein C cleavage.

Use Cases

• Modeling protease-dependent functional selectivity in GPCRs based on the comparison of thrombin and aPC cleavage.
• Analyzing Gαq versus Gα12/13 signaling axis divergence based on transducer-wide biosensor assay results.
• Identifying thrombin-responsive transcriptional elements based on the TRE-MPRA dataset analysis.
• Studying platelet activation markers like P-selectin expression and calcium flux based on primary human platelet experiments.

Strengths

• Dataset integrates multiple experimental methods, including biosensor assays, transcriptional analysis, and primary cell studies.
• Focuses on a specific endogenous receptor system (PAR1) as a model for functional selectivity.
• Published under a CC-BY-4.0 license, permitting broad reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The description is detailed but lacks explicit sample data or column names for direct inspection.

Provenance

Source

figshare

Collection Method

Integrated experimental results from biosensor assays (TRUPATH, TGFα shedding, PRESTO-Tango), transcriptional response element analysis (TRE-MPRA), and platelet activation studies.

Freshness

Last updated 2026-05-25 04:34:42; freshness should be verified.