Proteomic Analysis of Lysinibacillus PET Biodegradation Potential

A proteomic dataset from a study evaluating a PET-associated bacterial strain, Lysinibacillus sp., isolated from Icelandic soil. The data includes results from growth assays, proteomics, in silico screening, and surface imaging over a six-week period. It compares the strain's performance against the reference strain Ideonella sakaiensis.

Use Cases

• Analyze proteomic data to identify oxidative-stress response proteins like peroxiredoxins and superoxide dismutases expressed during PET exposure.
• Compare early-stage growth assay results between Lysinibacillus sp. and Ideonella sakaiensis across six weeks of observation.
• Use in silico genome-wide screening results to investigate the absence of canonical PET-degrading enzymes such as PETase or MHETase.
• Correlate FE-SEM surface imaging data showing erosion and delamination with proteomic expression profiles.

Strengths

• Includes a six-week time series of growth assay data comparing two bacterial strains.
• Combines multiple analytical methods: proteomics, in silico screening, and surface imaging (FE-SEM).
• Provides specific statistical significance (p < 0.05) for growth differences at multiple time points.

Limitations

• Dataset is small (5.8 MB), suggesting limited raw data volume.
• No canonical PET-degrading enzymes were detected, limiting direct applications for enzyme discovery.
• Proteomic analysis is specific to a single bacterial strain isolated from Icelandic soil, limiting generalizability.

Provenance

Source

Radoslaw B. Dudziak via figshare.

Collection Method

Bacterial strain isolation from Icelandic soil, followed by growth assays, proteomics (MALDI-TOF MS), in silico screening, and surface imaging (FE-SEM).

Time Range

Study period covers six weeks of growth assays.

Freshness

Last updated March 24, 2026.

Geography

Bacterial strain isolated from Icelandic soil.