Purkinje Cell AIS Knockout Study: Molecular and Transcriptional Analysis

A figshare-hosted research article by Qian Shi, last updated April 13, 2026. The 318 KB PDF details a study on Purkinje cell-specific knockout mice, investigating the roles of Neurofascin 186 and Ankyrin G in axon initial segment formation, synaptic organization, and neurodegeneration.

Use Cases

• Analyze gene expression patterns based on the RNA-seq data mentioned in the description
• Study protein localization and AIS disruption based on the described immunohistochemistry findings
• Model neurodegenerative progression based on the described timeline of Purkinje cell degeneration
• Investigate synaptic organization defects based on the described Kv1.2 clustering and basket cell axon misalignment

Strengths

• Data is openly shared under a CC-BY-4.0 license.
• The study provides a detailed molecular and transcriptional analysis of specific genetic knockouts.
• Research includes a defined postnatal developmental timeline from days 10 to 30 and up to 2 months.

Limitations

• The dataset is a single 318 KB PDF file; the underlying raw data (e.g., RNA-seq counts, microscopy images) is not directly provided.
• Column-level documentation for any supplementary data is absent; field semantics must be inferred from the paper.
• Row count for any potential tabular data is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Experimental study using Purkinje cell-specific knockout mice, immunohistochemistry, and RNA-seq analysis.

Time Range

Postnatal developmental stages (P10-P30) and up to 2 months of age in mice.

Freshness

Last updated 2026-04-13 04:35:19; freshness should be verified.

Geography

Laboratory study; geographic location is not specified.