Rat Model Data on SHPL-49 Effects in Ischemic Stroke

A 1.5 MB PDF document presents experimental data from a study on the salidroside derivative SHPL-49 in rats with bilateral common carotid artery occlusion. The research, authored by Pei Zhang, assesses cognitive and motor function improvements and analyzes synaptic remodeling via Golgi staining, Western blotting, and RNA sequencing. It investigates the CDK5/p35/p25 signaling pathway as a potential mechanism of action.

Use Cases

• Analyze behavioral performance metrics from Morris water maze, shuttle box, and rotarod tests to quantify SHPL-49's effect on cognitive and motor deficits.
• Correlate dendritic spine density measurements from Golgi staining with expression levels of synapse-related genes like MAP-2, SYP, and βIII-tubulin.
• Validate transcriptomic findings from PC-12 cell RNA sequencing by examining protein expression of CDK5, p35, and p25 in primary neuron and rat brain tissue.
• Investigate the relationship between the inhibition of p35 cleavage into p25 and improvements in synaptic protein SYN1 expression.

Strengths

• Data is derived from a controlled experimental model (BCCAO rats) with specific behavioral and molecular assessments.
• Includes multi-modal validation through in vivo behavioral tests, histological staining, protein analysis, and in vitro transcriptomics.
• Published under a permissive CC BY 4.0 license, facilitating reuse and sharing.

Limitations

• The dataset is contained within a single 1.5 MB PDF, limiting direct computational analysis of raw, structured data.
• Sample size for the rat model and in vitro experiments is not specified, which may affect statistical power assessment.
• Data is static and tied to a specific 2026 publication, with no indication of ongoing updates or expansions.

Provenance

Source

figshare, authored by Pei Zhang.

Collection Method

Experimental data generated from BCCAO rat models, primary neuron cultures, and PC-12 cell line transcriptomics.

Time Range

Study conducted over a 28-day treatment period; publication date is 2026.

Freshness

Last updated on 2026-03-23.

Geography

Laboratory study; geographic location of the research institution is not specified.