Secreted Proteins from Bacillus subtilis K1 Inducing Apple Resistance Against Gray Mold

A 187.1 KB PDF research article by Peiqian Li, last updated on 2026-05 04. The study investigates the molecular mechanisms of the antagonistic bacterium Bacillus subtilis K1 against Botrytis cinerea (gray mold) in apple fruit. It identifies a secreted protein, GM001344, which reduced disease incidence by 63.5% and improved postharvest fruit quality metrics like firmness and ascorbic acid content.

Use Cases

• Identifying candidate protein elicitors for biocontrol based on genomic bioinformatics screening mentioned in the description.
• Studying the induction of systemic resistance in plants based on the measurement of defense-related enzyme activities and malondialdehyde (MDA) content.
• Evaluating the effect of protein treatments on postharvest fruit quality based on metrics like firmness, total soluble solids, and ascorbic acid.
• Designing inoculation assays to test disease control efficacy based on the protocols for disease incidence and severity index reduction.

Strengths

• Includes specific quantitative results: GM001344 pretreatment reduced disease incidence to 63.5% versus 100% in control and lowered the disease severity index to 38.43 versus 76.75.
• Reports on multiple validated outcomes: enhanced defense enzyme activities, reduced MDA content, and improved postharvest quality metrics (firmness, total soluble solids, ascorbic acid) after 6 days.
• Provides a clear molecular mechanism focus, identifying a specific gene (GM001344) and its purified recombinant protein.

Limitations

• The dataset is a 187.1 KB PDF document; the underlying experimental data (e.g., raw enzyme activity readings, full protein sequences) is not directly accessible in a structured format.
• Row count and column-level documentation are absent; data semantics and structure must be inferred from the article text.
• The geographic and temporal scope of the underlying experiments is not specified in the provided metadata.

Provenance

Source

figshare, author Peiqing Li.

Collection Method

Laboratory experiments involving genomic analysis, gene cloning, transient expression in tobacco, protein purification, and apple fruit inoculation assays.

Time Range

The study period is not specified.

Freshness

Last updated 2026-05-04 05:30:30.

Geography

The geographic origin of the Bacillus subtilis K1 strain or the apple fruit is not specified.