Data Sheet 1_Serum-free production of anti-huCD20(hγ1)-IL2no-alpha immunocytokine: a promi

A research paper published on figshare in April 2026 details the serum-free production of a novel anti-huCD20(hγ1)-IL2no-alpha immunocytokine for B-cell non-Hodgkin lymphoma. Author Debbie Laura Bergantiños Albernas describes kinetic characterization of CHO cell clones, purification, and functional assays including B-cell depletion and antitumor efficacy in mice. The 406.9 KB PDF contains the full study methods and results.

Use Cases

• Analyzing cell growth and productivity kinetics in serum-free media based on the described batch and pseudo-perfusion experiments.
• Evaluating the impact of culture media on cell-cycle progression and mTOR signaling as mentioned in the kinetic characterization.
• Assessing the structural integrity and aggregation profile of purified immunocytokines compared to serum-containing cultures.
• Studying ex vivo B-cell depletion activity using PBMC from patients as described in the biological assays.
• Investigating antitumor efficacy in mouse models bearing EL4-hCD20- cells based on the reported in vivo results.

Strengths

• Data is openly available under a CC-BY-4.0 license.
• The description provides a detailed methodological overview of cell culture, purification, and functional assays.
• Research focuses on a novel therapeutic candidate for relapsed or refractory B-NHL.

Limitations

• The dataset is a 406.9 KB PDF; raw numerical or tabular data is not directly provided.
• Column-level documentation and sample data are unavailable, requiring extraction from the text.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare, author Debbie Laura Bergantiños Albernas.

Collection Method

Experimental research involving mammalian cell culture, protein purification, and biological assays.

Time Range

Study period not specified; publication date is 2026.

Freshness

Last updated 2026-04-15 11:48:18; freshness should be verified.

Geography

Geographic coverage of the study is not specified.