Ovine Seminal Plasma Biomarkers for Oxidative DNA Damage Quantified by LC-MS/MS

A dataset containing quantified levels of free nucleosides 2′-deoxyguanosine and 8-hydroxy-2′-deoxyguanosine in ovine seminal plasma, used as biomarkers for oxidative DNA damage. The data was generated by author Manuel Alfaro-Gómez using a validated solid-phase extraction and LC-MS/MS method, with results from a pooled healthy sample and one ram with low sperm quality. The dataset was last updated on May 8, 2026.

Use Cases

• Validate analytical methods for biomarker quantification based on the described SPE off-line LC-MS/MS protocol.
• Model the relationship between oxidative stress biomarkers and sperm quality conditions like asthenozoospermia or oligozoospermia based on the described proof-of-concept results.
• Assess the sustainability of bioanalytical methods using green analytical chemistry metrics like GAPI and AGREE as referenced in the description.

Strengths

• Analytical method validation followed US FDA guidelines for bioanalytical methods, assessing precision, linearity, LOD, LOQ, recoveries, and matrix effect.
• The dataset includes a direct comparison between a pooled healthy sample and a sample from a ram with low sperm quality, showing marked biomarker elevation.
• The dataset is licensed under CC-BY-4.0, permitting open sharing and adaptation.

Limitations

• Row count is unknown, which may limit suitability assessment for statistical modeling.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (2.7 MB), which may limit the scope of analysis.

Provenance

Source

figshare, author Manuel Alfaro-Gómez.

Collection Method

Data generated via solid-phase extraction and LC-MS/MS quantification of biomarkers in ovine seminal plasma.

Time Range

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Freshness

Last updated 2026-05-08 05:48:10; freshness should be verified.

Geography

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