Single-Cell RNA-Seq Data for Intervertebral Disc Degeneration MSC Analysis

Fengyu Ma published a dataset on figshare in 2026. It contains single-cell RNA sequencing data from 11 nucleus pulposus samples (8 diseased and 3 control) used to study mesenchymal stromal cells in intervertebral disc degeneration. The data was analyzed to derive extracellular vesicle-associated program scores and identify hub genes like AP2S1 and CSTB.

Use Cases

• Training models to classify diseased versus control samples based on derived MSC EV-program scores.
• Analyzing ligand-receptor communication networks between cell types using inferred CellChat data.
• Investigating correlations between hub gene expression and immune-metabolic pathway signatures.
• Prioritizing candidate biomarker genes for intervertebral disc degeneration using machine-learning workflows.

Strengths

• Includes 11 human samples (8 IDD, 3 control) for comparative analysis.
• Analysis identified a hub-gene model with an AUC of 0.836 for distinguishing IDD from control.
• Data is licensed under CC-BY-4.0 for open reuse.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (1.5 MB), which may indicate limited raw data or processed results only.

Provenance

Source

figshare

Collection Method

Single-cell RNA sequencing data from GSE230809, processed with standard preprocessing and batch correction.

Freshness

Last updated 2026-05-22 04:30:53; freshness should be verified.