Data Sheet 1_Specimen-specific differences in clinical metagenomic sequencing reporting pa

A retrospective observational study of 1,981 clinical metagenomic next-generation sequencing (mNGS) orders from a single center between January 1, 2024, and December 31, 2025. The data compares report-interpreted positivity and pathogen detection across bronchoalveolar lavage fluid, blood, cerebrospinal fluid, and tissue specimens. The dataset was authored by Jian-min Ren and shared under a CC-BY-4.0 license.

Use Cases

• Compare report-interpreted positivity rates based on specimen type (BALF, blood, CSF, tissue).
• Analyze the frequency of mixed pathogen detections among positive orders.
• Rank the most frequently reported organisms (e.g., Epstein-Barr virus, Candida albicans) across specimen groups.
• Stratify findings by ICU association as described in the study.

Strengths

• Includes 1,981 specimen-specific first orders with clear counts per type (e.g., 973 BALF).
• Reports specific positivity percentages with 95% confidence intervals for each specimen group.
• Distinguishes between DNA-only and a subset of 277 PMseq-RNA-tested orders for RNA virus analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 26.2 KB file size suggests a very limited scope, likely containing summary statistics rather than raw sequencing data.

Provenance

Source

figshare

Collection Method

Retrospective analysis of routine clinical mNGS orders from a single center.

Time Range

January 1, 2024 to December 31, 2025

Freshness

Last updated 2026-05-20 10:44:12; freshness should be verified.