Microglial and Neuronal Cell Responses to Commensal and Pathogenic LPS

A 526.4 KB PDF research article by Valentina Mazziotti, last updated April 2026, compares the inflammatory effects of lipopolysaccharides from Escherichia coli and commensal Phocaeicola vulgatus on microglial and neuronal cells. The study's data, shared under a CC-BY-4.0 license, includes measurements of nitric oxide, pro-inflammatory cytokines, TLR4 signaling, and neuronal integrity via immunofluorescence. Findings argue that neuroinflammatory outcomes are chemistry-dependent, challenging the universal use of LPS as a neuroinflammation model.

Use Cases

• Compare microglial activation profiles based on LPS chemotype as described in the study.
• Model neuron-glia interactions using the conditioned media transfer experimental paradigm outlined.
• Investigate TLR4 pathway specificity in response to different bacterial LPS structures.
• Benchmark in vitro neuroinflammation models against the commensal vs. pathogenic LPS data.

Strengths

• Data is from a controlled comparative analysis using both murine (BV2) and human (HMC3) microglial cell lines.
• Includes specific outcome measures like nitric oxide production, cytokine release (ELISA), and TLR4 signaling (western blot).
• Neuronal impact is assessed via cell viability and MAP2 expression in PC12 cells.

Limitations

• The dataset is a single 526.4 KB PDF file; underlying raw data tables or images are not separately provided.
• Row count and column-level documentation for any underlying data are unknown.
• The experimental scope is limited to in vitro cell culture models.

Provenance

Source

Valentina Mazziotti via figshare.

Collection Method

Data generated from laboratory experiments on BV2, HMC3, and PC12 cell lines, with analyses including ELISA, western blot, and immunofluorescence.

Freshness

Last updated 2026-04-14 04:14:22.