Study on Hinokitiol's Anti-Biofilm Mechanism Against Staphylococcus Epidermidis

A 2.0 MB PDF research article by Ting Zhang, last updated May 21, 2026, investigates the antibacterial and anti-biofilm activities of hinokitiol against Staphylococcus epidermidis. The study details mechanism experiments including checkerboard assays, membrane permeability tests, and transcriptomic analysis. It is licensed under CC-BY-4.0 and hosted on figshare.

Use Cases

• Analyze synergistic drug effects based on checkerboard assay results mentioned in the description
• Study bacterial membrane disruption mechanisms based on NPN uptake and PI staining data
• Investigate gene expression changes in biofilm formation pathways based on transcriptomic analysis of icaA, fnbA, and aap genes
• Assess cytotoxicity and hemolytic activity of potential therapeutics based on mammalian cell and sheep erythrocyte tests

Strengths

• The study includes multiple experimental methods: checkerboard assay, membrane permeability tests, and transcriptomic analysis.
• The description explicitly notes a significant synergistic effect (FICI ≤ 0.5) between hinokitiol and Ceftiofur sodium.
• Mechanistic insights are provided, including disruption of membrane permeability and induction of reactive oxygen species (ROS).

Limitations

• The dataset is a single PDF research article; the underlying raw experimental data (e.g., columnar results) are not provided separately.
• The study's limitations, as stated in the description, include the absence of in vivo mastitis models and pharmacokinetic data.
• Row count and column-level documentation are unknown, limiting suitability assessment for direct computational analysis.

Provenance

Source

figshare

Collection Method

Laboratory research study involving checkerboard assays, cytotoxicity tests, membrane permeability assays, and transcriptomic analysis.

Freshness

Last updated 2026-05-21 05:42:15.