Subcellular Localization of Digoxin Biosynthesis Enzymes in Foxglove

Digoxin, a widely prescribed cardiac drug, has a partially understood biosynthetic pathway. This research article identifies the subcellular localization of three key enzymes in Digitalis lanata, revealing endoplasmic reticulum and cytosol targeting. The findings were published by Viviane Zeng in March 2026.

Use Cases

• Inferring spatial organization of metabolic pathways based on enzyme localization data.
• Guiding metabolic engineering strategies for plant-based pharmaceuticals based on the described subcellular compartments.
• Comparing plant and mammalian steroid biosynthesis based on the differing localization of the P450scc enzyme.

Strengths

• Focuses on three specific, medically important enzymes: P450scc, 3βHSD, and P5βR2.
• Provides a direct comparison to mammalian enzyme localization, highlighting a key biological difference.
• Released under a permissive CC-BY-4.0 license for reuse.

Limitations

• The dataset is a 1.9 MB PDF; the underlying experimental data (e.g., microscopy images, quantitative measurements) is not provided in a structured, machine-readable format.
• Row count and column-level documentation are unknown, limiting suitability assessment for computational analysis.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, author Viviane Zeng.

Collection Method

Experimental study using fluorescent tagging and expression in tobacco leaves.

Time Range

Publication date March 2026.

Freshness

Last updated 2026-03-18 07:44:19.

Geography

Study focuses on the plant Digitalis lanata (foxglove).