Rice GA2ox Gene Family Mutant Phenotypes from Systematic CRISPR/Cas9 Editing

Kun-Ting Hsieh's research dataset details phenotypic analyses of 36 CRISPR/Cas9-edited rice lines targeting nine OsGA2ox genes. The data, published on figshare in 2026, includes single-, double-, triple-, and quadruple-gene knockout mutants. It systematically compares gene-specific and combinatorial functions on agronomic traits like plant height, grain number, and fertility.

Use Cases

• Identify dominant and minor gene paralogs based on mutant phenotype severity described in the results.
• Model hierarchical regulatory frameworks within gene families based on combinatorial mutant analysis.
• Investigate trade-offs between plant growth and reproductive traits using data on height increases versus reduced grain weight/fertility.
• Compare functional redundancy patterns across different gene classes (I, II, III) as outlined in the study.

Strengths

• Includes data from 36 distinct CRISPR/Cas9-edited mutant lines.
• Targets all nine members of the OsGA2ox gene family for systematic comparison.
• Analyzes combinatorial effects through double, triple, and quadruple mutants.
• Published under a permissive CC-BY-4.0 license for reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is contained within a 1.8 MB PDF, which may require extraction for computational analysis.

Provenance

Source

Kun-Ting Hsieh via figshare.

Collection Method

Generated through CRISPR/Cas9 gene editing and systematic phenotypic analysis of rice mutants.

Freshness

Last updated 2026-05-07 05:42:22; freshness should be verified.